Janet Lovett

Janet Eleanor Lovett

Postal address:
School of Physics & Astronomy
Physical Science Building
North Haugh
St Andrews
United Kingdom

E-mail: jel20@st-andrews.ac.uk

Direct phone: +44 (0)1334 463120

Research overview

Measuring nanometre distances for structural biology using EPR spectroscopy.

I use Electron Paramagnetic Resonance (EPR) spectroscopy to measure nanometre distances in proteins and nucleic acids. This provides detail on their structure and dynamics. The methodology is applied to a range of proteins including those for protein excretion in the cell. I am also interested in developing the methods for tagging proteins and nucleic acids to make them suitable for this kind of investigation, as well as advancing the EPR experiments themselves.

The biological preparation and EPR facilities at St Andrews are outstanding with, among others, pulsed and CW X-band, high power Q-band and the home-built high power W-band (HiPER).

Recent Publications

The use of composite pulses for improving DEER signal at 94 GHz
Motion, C. L., Cassidy, S. L., Cruickshank, P. A. S., Hunter, R. I., Bolton, D. R., El Mkami, H., Van Doorslaer, S., Lovett, J. E. & Smith, G. M. May 2017 In : Journal of Magnetic Resonance. 278, p. 122-133 12 p.
Research output: Contribution to journalArticle

Tuning the properties of nitroxide spin labels for use in electron paramagnetic resonance spectroscopy through chemical modification of the nitroxide framework
Haugland, M., Anderson, E. A. & Lovett, J. E. Dec 2016 Electron Paramagnetic Resonance. Chechik, V. & Murphy, D. (eds.). RSC, Vol. 25, p. 1-34 (Special Periodical Reports; vol. 25)
Research output: Chapter in Book/Report/Conference proceedingChapter

Synthesis of next generation maleimide radical labels
Hajjaj, B., Shah, A., Bell, S., Shirran, S. L., Botting, C. H., Slawin, A. M. Z., Hulme, A. & Lovett, J. E. Oct 2016 In : Synlett. p. 2357-2361 5 p., 27
Research output: Contribution to journalArticle

2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA
Haugland, M. M., El-Sagheer, A. H., Porter, R. J., Peña, J., Brown, T., Anderson, E. A. & Lovett, J. E. 27 Jul 2016 In : Journal of the American Chemical Society. 138, 29, p. 9069-9072 4 p.
Research output: Contribution to journalArticle

DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR
Motion, C. L., Lovett, J. E., Bell, S., Cassidy, S. L., Cruickshank, P. A. S., Bolton, D. R., Hunter, R. I., El Mkami, H., Van Doorslaer, S. & Smith, G. M. Apr 2016 In : Journal of Physical Chemistry Letters. 7, 8, p. 1411-1415
Research output: Contribution to journalArticle

Exploiting orientation-selective DEER: determining molecular structure in systems containing Cu(II) centres
Bowen, A. M., Jones, M. W., Lovett, J. E., Gaule, T. G., McPherson, M. J., Dilworth, J. R., Timmel, C. R. & Harmer, J. R. 28 Feb 2016 In : Physical Chemistry Chemical Physics. 18, 8, p. 5981-5994 14 p.
Research output: Contribution to journalArticle

Investigating the structure of the factor B vWF-A domain/CD55 protein-protein complex using DEER spectroscopy: successes and pitfalls
Lovett, J. E., Abbott, R. J. M., Roversi, P., Johnson, S., Caesar, J. J. E., Doria, M., Jeschke, G., Timmel, C. R. & Lea, S. M. 1 Oct 2013 In : Molecular Physics. 111, 18-19, p. 2865-2872 8 p.
Research output: Contribution to journalArticle

DEER-Stitch: Combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data
Lovett, J. E., Lovett, B. W. & Harmer, J. Oct 2012 In : Journal of Magnetic Resonance. 223, p. 98-106 9 p.
Research output: Contribution to journalArticle

Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies
Orwick-Rydmark, M., Lovett, J. E., Graziadei, A., Lindholm, L., Hicks, M. R. & Watts, A. Sep 2012 In : Nano Letters. 12, 9, p. 4687-4692 6 p.
Research output: Contribution to journalArticle

Structural changes caused by radiation-induced reduction and radiolysis: the effect of X-ray absorbed dose in a fungal multicopper oxidase
De la Mora, E., Lovett, J. E., Blanford, C. F., Garman, E. F., Valderrama, B. & Rudino-Pinera, E. May 2012 In : Acta Crystallographica. Section D, Biological crystallography. 68, p. 564-577 14 p.
Research output: Contribution to journalArticle

The Short-Lived Signaling State of the Photoactive Yellow Protein Photoreceptor Revealed by Combined Structural Probes
Ramachandran, P. L., Lovett, J. E., Carl, P. J., Cammarata, M., Lee, J. H., Jung, Y. O., Ihee, H., Timmel, C. R. & van Thor, J. J. 22 Jun 2011 In : Journal of the American Chemical Society. 133, 24, p. 9395-9404 10 p.
Research output: Contribution to journalArticle

Shigella flexneri Spa15 Crystal Structure Verified in Solution by Double Electron Electron Resonance
Lillington, J. E. D., Lovett, J. E., Johnson, S., Roversi, P., Timmel, C. R. & Lea, S. M. 14 Jan 2011 In : Journal of Molecular Biology. 405, 2, p. 427-435 9 p.
Research output: Contribution to journalArticle

Probing Flexibility in Porphyrin-Based Molecular Wires Using Double Electron Electron Resonance
Lovett, J. E., Hoffmann, M., Cnossen, A., Shutter, A. T. J., Hogben, H. J., Warren, J. E., Pascu, S. I., Kay, C. W. M., Timmel, C. R. & Anderson, H. L. 30 Sep 2009 In : Journal of the American Chemical Society. 131, 38, p. 13852-13859 8 p.
Research output: Contribution to journalArticle

Neisseria meningitidis recruits factor H using protein mimicry of host carbohydrates
Schneider, M. C., Prosser, B. E., Caesar, J. J. E., Kugelberg, E., Li, S., Zhang, Q., Quoraishi, S., Lovett, J. E., Deane, J. E., Sim, R. B., Roversi, P., Johnson, S., Tang, C. M. & Lea, S. M. 16 Apr 2009 In : Nature. 458, 7240, p. 890-U9 6 p.
Research output: Contribution to journalArticle

Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase
Barry, E. R., Lovett, J. E., Costa, A., Lea, S. M. & Bell, S. D. 27 Jan 2009 In : Proceedings of the National Academy of Sciences of the United States of America. 106, 4, p. 1051-1056 6 p.
Research output: Contribution to journalArticle

Structural information from orientationally selective DEER spectroscopy
Lovett, J. E., Bowen, A. M., Timmel, C. R., Jones, M. W., Dilworth, J. R., Caprotti, D., Bell, S. G., Wong, L. L. & Harmer, J. 2009 In : Physical Chemistry Chemical Physics. 11, 31, p. 6840-6848 9 p.
Research output: Contribution to journalArticle

Determining the structure of the complex between vWF-A of factor B and CD55
Banham, J. E., Abbott, R., Caesar, J., Roversi, P., Jeschke, G., Timmel, C. & Lea, S. Oct 2008 In : Molecular Immunology. 45, 16, p. 4122-4122 1 p.
Research output: Contribution to journalAbstract

Distance measurements in the borderline region of applicability of CW EPR and DEER: A model study on a homologous series of spin-labelled peptides
Banham, J. E., Baker, C. M., Ceola, S., Day, I. J., Grant, G. H., Groenen, E. J. J., Rodgers, C. T., Jeschke, G. & Timmel, C. R. Apr 2008 In : Journal of Magnetic Resonance. 191, 2, p. 202-218 17 p.
Research output: Contribution to journalArticle

Evidence from EPR that nitroxide spin labels attached to human hemoglobin alter their conformation upon freezing
Banham, J. E., Jeschke, G. & Timmel, C. R. 24 Aug 2007 In : Molecular Physics. 105, 15-16, p. 2041-2047 7 p.
Research output: Contribution to journalArticle

DeerAnalysis2006 - a comprehensive software package for analyzing pulsed ELDOR data
Jeschke, G., Chechik, V., Ionita, P., Godt, A., Zimmermann, H., Banham, J. E., Timmel, C. R., Hilger, D. & Jung, H. 2006 In : Applied Magnetic Resonance. 30, 3-4, p. 473-498 26 p.
Research output: Contribution to journalArticle

The characterization of weak protein-protein interactions: Evidence from DEER for the trimerization of a von Willebrand factor A domain in solution
Jeschke, G., Abbott, R. J. M., Lea, S. M., Timmel, C. R. & Banham, J. E. 2006 In : Angewandte Chemie International Edition. 45, 7, p. 1058-1061 4 p.
Research output: Contribution to journalArticle